monoclonal antibody and a monoclonal antibody-enzyme conjugate directed at different antigenic sites. This results in the collagen IV molecule being sandwiched between the solid phase... and enzyme labelled antibodies. After removing unbound enzyme labelled antibody and sample, the plate is then incubated with enzyme substrate, resulting in the development...

for the assay of urinary collagen IV. It is a solid phase one-step sandwich immunoassay. Collagen IV in the sample is bound simultaneously by a solid phase monoclonal antibody... and a monoclonal antibody-enzyme conjugate directed at different antigenic sites. This results in the collagen IV molecule being sandwiched between the solid phase and enzyme labelled... antibodies. After removing unbound enzyme labelled antibody and sample, the plate is then incubated with enzyme substrate, resulting in the development of a color. The color developed...

of plate Incorrect shaking motion can affect proper mixing and antibody/analyte interaction. Due to various equipment models please refer to the Learning Center section... for the assay. Inadequate shaking of plate Incorrect shaking motion can affect proper mixing and antibody/analyte interaction. Due to various equipment models please refer to the Learning.... In flow cytometry, fluorophores are used to tag antibodies in order to provide a signal upon detection of an antigen on the sample when exposed to a single wavelength laser. How...

of plate Incorrect shaking motion can affect proper mixing and antibody/analyte interaction. Due to various equipment models please refer to the Learning Center section of the website.... Inadequate shaking of plate Incorrect shaking motion can affect proper mixing and antibody/analyte interaction. Due to various equipment models please refer to the Learning Center... flow cytometry, fluorophores are used to tag antibodies in order to provide a signal upon detection of an antigen on the sample when exposed to a single wavelength laser. How...