downstream, the dispersion process mixes sample with reagent forming a reaction product (yellow). The extent of mixing and the length of reaction time is controlled by the flow rate... are individually processed in exactly the same way, the calibration curve is valid for unknown samples to be assayed The peak height recorded by the detector is proportional to the analyte... protocol, sample injection, mixing, incubation, product monitoring and instrument washout is best performed at an different flow rate. Thus instrument washout is most efficiently...