in order to simulate the actual samples to be analyzed. For example, if your samples contain a known sodium background such as 150 millimoles NaCl then your calibrating standards should... on semilog paper (See Sample Calibration Curve). Remember! For best results, do the following: Always use standards that bracket the concentration range that you expect to have in your... samples. Always mix your standards to closely match the composition of your samples. Always calibrate using the same volume of standard that you will have as a sample. Example...