in disaggregation. The high pressures used by PreEMT forces the protein into its most compact form, usually its native state. Biologically active, native protein is recovered within hours... at yields as high as 100%, even at high protein concentrations. PreEMT can be applied to a wide variety of therapeutic proteins (existing or novel), including single polypeptide chains..., yeast or E. coli. PreEMT has significant utility in the refolding of recombinant proteins produced in E. coli at high expression levels as inclusion bodies, most particularly...

Publications Events Protein Therapeutics PreEMT is a patented technology for the disaggregation and controlled refolding of proteins with high fidelity of native structure at yields... High Pressure Protein Refolding Technology 11/13/2012 Nuron Biotech Completes Enrollment of a Pivotal Phase 3 Study for Proprietary Recombinant Human Interferon Beta-1b (NU100... and efficiencies not always achievable using existing technologies. PreEMT is applicable to a wide variety of therapeutic proteins (existing or novel) including single polypeptide...

be life-threatening. Regulatory authorities have recently proposed reducing levels of protein aggregates in therapeutic products to minimize these immunogenicity concerns. High... compositions, which facilitates life-cycle extension strategies. Preliminary studies have also shown that high pressure treatment can facilitate protein crystal formation, resulting... Publications Events Our Technology Novel Biologics Fab Platform Follow-on Biologics Life-cycle Management Life-cycle Management Protein aggregates in final drug product...

Releases Publications Events High Pressure Refolding Window BaroFold provides client services in the area of protein refolding, aggregate characterization, formulation studies..., and microbial expression, centered around its proprietary PreEMT technology. Client services supported by BaroFold include:PROTEIN REFOLDINGBaroFold conducts high-pressure refolding... feasibility studies on all proteins, whether expressed from mammalian cells or E. coli. Having refolded approximately 100 proteins to date, BaroFold is highly knowledgeable in all...

therapeutics for bio-pharmaceutical companies, research institutions, and government agencies. PreEMT employs high pressure to disaggregate and refold proteins, which may have... Publications Events BaroFold applies its Pressure Enabled Protein Manufacturing (PreEMT ) technology to improve the tolerability, efficacy and safety of a wide variety of protein... a significant impact on the quality of these biotherapeutics by improving their activity, homogeneity and safety. BaroFold also helps companies create novel protein therapeutics...