Transfection Example of a Transfection (Transfection in the presence of 10% fetal bovine serum using NovaFECTOR) 1. Grow cells in 35mm dish using a medium that contains 10% fetal... bovine serum to 55-70% confluence. 2. Aspirate old medium from dish. Add plasmid DNA and NovaFECTOR lipid to 1.0 ml of serum free medium (in 1.5 ml siliconized microtube). Let sit... at room temperature for 15 minutes to allow DNA:lip complex formation. (Start with 2-3 ug of DNA and 8-10 ug of lipid, then change the concentration of DNA and/or lipid to optimize...

Venn Nova, Inc - Rewarding Science (TM) Superb performance at reasonable prices! Welcome to Venn Nova, Inc., home of the best lipids for DNA transfection. Venn Nova offers its... line of powerful polycationic liposome reagents for DNA transfection at the lowest prices in the market. These reagents give high transfection efficiencies with minimal or no toxicity...: Chick retinal cells transfected with GFP (green) using GeneFECTOR. Staining with an antibody to neurofilaments (red) demonstrates that the transfected cells are neurons. Home...

a constant number of cells to obtain the highest transfection efficiency. This is easily achieved starting with a constant amount of DNA (for example, 1 g per 35 mm dish) and varying... 0.5 g/0.5 g, 1.0 g/1.0 g, 1.5 g/1.5 g and 2.0 g/2.0 g total amounts of DNA/liposome complex in the second experiment. Transfection Time: Transfection efficiency is related... to the exposure time of the cells to the DNA/liposome complex. In general, the longer the exposure time the higher the efficiency. However, since transfection is carried out in serum free...