by the sequential action of at least three enzymes, ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin protein ligase (E3). E1 activates ubiquitin... and forms a thioester conjugate with activated ubiquitin, which is then transferred to E2. E3 interacts with both E2 and specific substrate, catalyzing the formation of an isopeptide... and hundreds of E3s, but only one essential E1. Therefore, E1 is the only common step in the ubiquitination process. While proteasome inhibitor prevents the degradative function...

includes the following assays: E1 thioester assay E2 thioester assay E3 auto-ubiquitination assay E3-mediated substrate ubiquitination assay Please contact us at Support for more... Products Multi-i Controls Custom Multi-i shRNA Vector Custom Lv-shRNA Vector References FAQ Ubiq E1 Introduction UBEI-41 Ubiq Evaluation References FQA Drug Leads Introduction...